H00001024-M03

antibody from Abnova Corporation

Targeting: CDK8 K35

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001024-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001024-M03, RRID:AB_529992

Product name

CDK8 monoclonal antibody (M03), clone 5H4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CDK8.

Antigen sequence

QQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPP
TTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSS
MGYSATSQQPPQYSHQTHRY


Isotype

IgG

Antibody clone number

5H4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in K-562 ( Cat # L009V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CDK8 monoclonal antibody (M03), clone 5H4. Western Blot analysis of CDK8 expression in Raw 264.7 ( Cat # L024V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CDK8 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol