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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- R20242 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H2BK23ac Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant H2BK23ac antibody is suitable for use in Western blot/ELISA/Immunocytochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM260
- Vial size
- 25 ug, 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H2BK23ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using recombinant H2BK23ac antibody at 0.5 ug/ml.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF of HeLa cells treated with sodium butyrate, using recombinant H2BK23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- This recombinant H2BK23ac antibody specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), 11 (K11ac), 12 (K12ac), 15 (K15ac), 20 (K20ac), or non-modified Lysine 23 in Histone H2B.
