GTX108166

antibody from GeneTex

Targeting: ESRRA ERR1, ERRa, ERRalpha, ESRL1, NR3B1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX108166

Provider

GeneTex

Proper citation

GeneTex Cat#GTX108166, RRID:AB_1950230

Product name

ERR alpha antibody [N1], N-term

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Estrogen-related receptor α is essential for maintaining mitochondrial integrity in cisplatin-induced acute kidney injury.

Tsushida K, Tanabe K, Masuda K, Tanimura S, Miyake H, Arata Y, Sugiyama H, Wada J
Biochemical and biophysical research communications 2018 Apr 15;498(4):918-924

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ERRα protein is stabilized by LSD1 in a demethylation-independent manner.

Carnesecchi J, Cerutti C, Vanacker JM, Forcet C
PloS one 2017;12(11):e0188871

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ERRα Maintains Mitochondrial Oxidative Metabolism and Constitutes an Actionable Target in PGC1α-Elevated Melanomas.

Luo C, Balsa E, Thomas A, Hatting M, Jedrychowski M, Gygi SP, Widlund HR, Puigserver P
Molecular cancer research : MCR 2017 Oct;15(10):1366-1375

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ERRα induces H3K9 demethylation by LSD1 to promote cell invasion.

Carnesecchi J, Forcet C, Zhang L, Tribollet V, Barenton B, Boudra R, Cerutti C, Billas IM, Sérandour AA, Carroll JS, Beaudoin C, Vanacker JM
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 11;114(15):3909-3914

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miR-135a Inhibits the Invasion of Cancer Cells via Suppression of ERRα.

Tribollet V, Barenton B, Kroiss A, Vincent S, Zhang L, Forcet C, Cerutti C, Périan S, Allioli N, Samarut J, Vanacker JM
PloS one 2016;11(5):e0156445

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Estrogens induce rapid cytoskeleton re-organization in human dermal fibroblasts via the non-classical receptor GPR30.

Carnesecchi J, Malbouyres M, de Mets R, Balland M, Beauchef G, Vié K, Chamot C, Lionnet C, Ruggiero F, Vanacker JM
PloS one 2015;10(3):e0120672

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Estrogen-related receptor α decreases RHOA stability to induce orientated cell migration.

Sailland J, Tribollet V, Forcet C, Billon C, Barenton B, Carnesecchi J, Bachmann A, Gauthier KC, Yu S, Giguère V, Chan FL, Vanacker JM
Proceedings of the National Academy of Sciences of the United States of America 2014 Oct 21;111(42):15108-13

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Estrogen Receptor α (ERα) and Estrogen Related Receptor α (ERRα) are both transcriptional regulators of the Runx2-I isoform.

Kammerer M, Gutzwiller S, Stauffer D, Delhon I, Seltenmeyer Y, Fournier B
Molecular and cellular endocrinology 2013 Apr 30;369(1-2):150-60

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Regulation of glycolysis and the Warburg effect by estrogen-related receptors.

Cai Q, Lin T, Kamarajugadda S, Lu J
Oncogene 2013 Apr 18;32(16):2079-86

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ?g of whole cell lysate)A:293TB:A431(GTX27909)C:H129912% SDS PAGEGTX108166 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERR alpha antibody [N1], N-term detects ERR alpha protein by western blot analysis.A. 50 ?g mouse kideny extract10% SDS-PAGEERR alpha antibody [N1], N-term (GTX108166) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERR alpha antibody [N1], N-term detects ERR alpha protein by western blot analysis.A. 50 ?g rat kideny extract10% SDS-PAGEERR alpha antibody [N1], N-term (GTX108166) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of ERR alpha protein by 2 £gg of ERR alpha antibody (GTX108166) 7.5% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (GTX108166) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ERR alpha antibody [N1], N-term detects ERR alpha protein at nucleus on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ERR alpha antibody [N1], N-term (GTX108166) diluted at 1:500.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 £gg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.