MA5-32194

antibody from Invitrogen Antibodies

Targeting: ESRRA ERR1, ERRa, ERRalpha, ESRL1, NR3B1

Western blot

Antibody data

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Validation data

Product number: MA5-32194 - Provider product page
Provider: Invitrogen Antibodies
Product name: ESRRA Recombinant Rabbit Monoclonal Antibody (SC57-07)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Antibody clone number: SC57-07
Vial size: 100 μL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ESRRA protein structure - MA5-32194 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded mouse brain. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded human kidney. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded rat brain. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded rat kidney. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded human brain. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded mouse kidney. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of ESRRA with paraffin-embedded mouse hippocampus. The tissues were blocked in 1% BSA (20 min, room temp) washed with ddH2O and PBS, incubated with recombinant monoclonal ESRRA (Product # MA5-32194) at a dilution of 1:1,000 (1 hr, room temperature). Detection was performed with HRP, DAB was used as the chromogen, counterstained with hematoxylin and mounted with DPX.