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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-28390 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ESRRA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T. Predicted reactivity: Mouse (98%), Rat (98%), Dog (98%), Pig (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.93 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Increased ABCC4 Expression Induced by ERRĪ± Leads to Docetaxel Resistance via Efflux of Docetaxel in Prostate Cancer.
Huang H, Li J, Shen J, Lin L, Wu X, Xiang S, Li Y, Xu Y, Zhao Q, Zhao Y, Kaboli PJ, Li M, Li X, Wang W, Wen Q, Xiao Z
Frontiers in oncology 2020;10:1474
Frontiers in oncology 2020;10:1474
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ESRRA Polyclonal Antibody detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human intestine. ERR alpha stained by ESRRA Polyclonal Antibody (Product # PA5-28390) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 µg of either control rabbit IgG or ESRRA Polyclonal Antibody (Product # PA5-28390). The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 µg of ERR alpha antibody (Product # PA5-28390) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (Product # PA5-28390) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Direct interaction between ERRalpha and ABCC4. (A) Representative graph of ABCC4 promoter regions containing putative ERRalpha binding sites. (B) P1 and P3 regions were pulled down by ERRalpha antibody by CHIP assay. (C) Luciferase signal for plasmid-containing P1 region was increased by ERRalpha. (D) Luciferase signal for plasmid-containing P3 region was increased by ERRalpha. ** p < 0.01 compared with respective control.
