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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-28390 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ESRRA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T.
- Concentration
- 0.93 mg/mL
Submitted references Increased ABCC4 Expression Induced by ERRĪ± Leads to Docetaxel Resistance via Efflux of Docetaxel in Prostate Cancer.
Huang H, Li J, Shen J, Lin L, Wu X, Xiang S, Li Y, Xu Y, Zhao Q, Zhao Y, Kaboli PJ, Li M, Li X, Wang W, Wen Q, Xiao Z
Frontiers in oncology 2020;10:1474
Frontiers in oncology 2020;10:1474
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ESRRA Polyclonal Antibody (Product # PA5-28390). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. ESRRA Polyclonal Antibody (Product # PA5-28390) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ESRRA was achieved by transfecting SK-BR-3 with ESRRA specific siRNA (Silencer® select Product # s4831). Western blot analysis (Fig. a) was performed using whole cell extracts from the ESRRA knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ESRRA Polyclonal Antibody (Product # PA5-28390, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ESRRA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-ESRRA Polyclonal Antibody (Product # PA5-28390) and a 50 kDa band corresponding to ESRRA was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of SK-BR-3 (Lane 1), MCF7 (Lane 2), T-47D (Lane 3) and MCF 10A (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ESRRA Polyclonal Antibody detects ERR alpha protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human intestine. ERR alpha stained by ESRRA Polyclonal Antibody (Product # PA5-28390) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 µg of either control rabbit IgG or ESRRA Polyclonal Antibody (Product # PA5-28390). The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERR alpha antibody immunoprecipitates ERR alpha protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of ERR alpha protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of ERR alpha by 2.5 µg of ERR alpha antibody (Product # PA5-28390) 10% SDS-PAGE The immunoprecipitated ERR alpha protein was detected by ERR alpha antibody (Product # PA5-28390) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Direct interaction between ERRalpha and ABCC4. (A) Representative graph of ABCC4 promoter regions containing putative ERRalpha binding sites. (B) P1 and P3 regions were pulled down by ERRalpha antibody by CHIP assay. (C) Luciferase signal for plasmid-containing P1 region was increased by ERRalpha. (D) Luciferase signal for plasmid-containing P3 region was increased by ERRalpha. ** p < 0.01 compared with respective control.
