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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-451-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL4/MIP-1 beta Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects CCL4/MIP-1 beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) MIP-1 beta and recombinant mouse (rm) MIP-1 alpha is observed. This antibody will not neutralize the biological activity of rmMIP-1 alpha . In the chemotaxis assay this antibody will partially neutralize rhMIP-1 alpha and rhMIP-1 beta at a 10-30 fold higher IgG concentration.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q5QNV9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M
Scientific reports 2017 Apr 20;7:46514
Scientific reports 2017 Apr 20;7:46514
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
The Journal of experimental medicine 2008 Sep 1;205(9):2075-84
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6384-93
CCL4 protects from type 1 diabetes by altering islet beta-cell-targeted inflammatory responses.
Meagher C, Arreaza G, Peters A, Strathdee CA, Gilbert PA, Mi QS, Santamaria P, Dekaban GA, Delovitch TL
Diabetes 2007 Mar;56(3):809-17
Diabetes 2007 Mar;56(3):809-17
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
CC chemokines mediate leukocyte trafficking into the central nervous system during murine neurocysticercosis: role of gamma delta T cells in amplification of the host immune response.
Cardona AE, Gonzalez PA, Teale JM
Infection and immunity 2003 May;71(5):2634-42
Infection and immunity 2003 May;71(5):2634-42
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 1;164(5):2650-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CCL4/MIP-1 beta by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL4/MIP-1 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL4/MIP-1 beta in RAW 264.7 Mouse Cell Line. CCL4/MIP-1 beta was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line untreated (right panel) and treated with LPS (left panel) using Goat Anti-Mouse CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL4/MIP-1 beta in Mouse Small Intestine. CCL4/MIP-1 beta was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to Peyer's patches. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL4/MIP-1 beta and Neutral-ization by Mouse CCL4/ MIP-1 beta Antibody. Recombinant Mouse CCL4/MIP-1 beta (Catalog # 451-MB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL4/ MIP-1 beta (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL4/MIP-1 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-451-NA). The ND50 is typically 0.2-1.0 µg/mL.
