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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP2172a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2172a, RRID:AB_2143605
- Product name
- Mib1/Mindbomb Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- MIB Antibody (K28) (Cat. #AP2172a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MIB antibody detected the MIB protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) (Cat # AP2172a) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark."
- Primary Ab dilution
- 1:20~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
