PA5-47000

antibody from Invitrogen Antibodies

Targeting: CCL3 G0S19-1, LD78ALPHA, MIP-1-alpha, SCYA3

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47000

Provider

Invitrogen Antibodies

Product name

CCL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant human (rh) MIP-1 beta, rhMIP-1 gamma, rhMIP-3 alpha, rhMIP-3 beta, and recombinant mouse (rm) MIP-1 alpha is observed. This antibody neutralizes the biological activity of recombinant human CCL3/MIP-1 alpha. In the chemotaxis assay this antibody will also partially neutralize the biological activity of rhMIP-1 beta at a 20 fold higher IgG concentration. This antibody will not neutralize the biological activity of rmMIP-1 beta or rmMIP-1 alpha. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CCL3 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated in CCL3 polyclonal antibody (Product # PA5-47000) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CCL3 is shown by the decrease in mean RFU (relative fluorescent units - measured by Resazurin - measure of chemotaxis) with increasing concentrations of CCL3 Polyclonal Antibody (PA5-47000).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CCL3 in BaF3 mouse pro‚B cell line transfected with human CCR5. Samples were incubated in CCL3 polyclonal antibody (Product # PA5-47000). Recombinant Human CCL3/MIP‚1α chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant HumanCCL3/MIP‚1α (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP‚1α Antigen Affinity‚purified Polyclonal Antibody. The ND50 is typically 2-8 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of CCL3 in BaF3 mouse pro‚B cell line transfected with human CCR5. Samples were incubated in CCL3 polyclonal antibody (Product # PA5-47000). Recombinant Human CCL3/MIP‚1α chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant HumanCCL3/MIP‚1α (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL3/MIP‚1α Antigen Affinity‚purified Polyclonal Antibody. The ND50 is typically 2-8 µg/mL.