Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- RQ7351 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NCOA4 Antibody / Nuclear receptor coactivator 4
- Antibody type
- Polyclonal
- Description
- This highly specific NCOA4 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the NCOA4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) 293T, 2) PC-3, 3) HeLa, 4) HL60, 5) HepG2, 6) Daudi and 7) A431 cell lysate with NCOA4 antibody. Expected molecular weight ~70 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) rat RH35, 3) mouse liver and 4) mouse HEPA1-6 cell lysate with NCOA4 antibody. Expected molecular weight ~70 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HL60 cells with NCOA4 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= NCOA4 antibody.
