12533-1-AP

antibody from Proteintech Group

Targeting: HNF1B HNF1beta, LFB3, MODY5, TCF2, VHNF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12533-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12533-1-AP, RRID:AB_2116758

Product name

HNF1B antibody

Antibody type

Polyclonal

Description

KD/KO validated HNF1B antibody (Cat. #12533-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Loss of Brg1 and Pten in Pancreatic Ductal Cells Forms Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway.

Iimori K, Fukuda A, Nagao M, Matsuyama S, Ikeda M, Masui Y, Nakayama S, Aoyama N, Mizukoshi K, Kawai M, Yamakawa G, Iwane K, Omatsu M, Muta Y, Maruno T, Minaga K, Kuriyama K, Yazumi S, Araki O, Yamauchi Y, Anazawa T, Nagai K, Ferrer J, Nakanishi Y, Tsuruyama T, Hatano E, Seno H
Cellular and molecular gastroenterology and hepatology 2026;20(2):101639

---

SAMHD1 depletion restricts SARS-CoV-2 infection by suppressing HNF1-dependent ACE2 expression in lung epithelial cells.

Cheung PH, Chan P, Yang H, Ambrus K, Honne S, Kim B, Perlman S, Wu L
PLoS pathogens 2026 Mar;22(3):e1013659

---

Functional Difference of MYC and MYCN in Combined Hepatocellular-Cholangiocarcinoma: Regulation of Differentiation by HNF1B.

Goto M, Yamamoto M, Tanaka H, Fujii Y, Takasawa K, Kamikokura Y, Kamikokura M, Kobayashi N, Murakami T, Nishikawa Y, Takasawa A
Cancer science 2026 Feb;117(2):536-547

---

Engineered Endometrial Clear Cell Cancer-on-a-Chip Reveals Early Invasion-Metastasis Cascade of Cancer Cells.

Li C, Pan J, Shi Z, Zeng X, Xia X, He X, Wang W, Qiu B, Ding W, Huang D
Biomaterials research 2025;29:0177

---

SAMHD1 promotes SARS-CoV-2 infection by enhancing HNF1-dependent ACE2 expression in lung epithelial cells.

Cheung PH, Chan P, Yang H, Honne S, Kim B, Perlman S, Wu L
bioRxiv : the preprint server for biology 2025 Oct 26;

---

Identification of hepatocyte-primed cholangiocytes in the homeostatic liver by in vivo lentiviral gene transfer to mice and non-human primates.

Milani M, Starinieri F, Fabiano A, Beretta S, Plati T, Canepari C, Biffi M, Russo F, Berno V, Norata R, Sanvito F, Merelli I, Aloia L, Huch M, Naldini L, Cantore A
Cell reports 2025 Mar 25;44(3):115341

---

Prognostic value of protein expression, tumor morphology and location within the pancreas in pancreatic ductal adenocarcinoma.

Nishida H, Ando K, Kaimori R, Kawamura K, Daa T
Oncology letters 2025 Jun;29(6):288

---

Pancreatic β-cell regeneration in situ by the ALK3 agonist THR-123.

Álvarez-Cubela S, Altilio ID, Doke M, Klein D, Tamayo A, Alcázar Ó, Santana CG, Qadir MMF, Alver CG, Cruz F, Biggs O, Tovar Castro JD, Navarro-Rubio B, Ricordi C, Caicedo A, Buchwald P, Agarwal A, Oltra E, Pastori RL, Domínguez-Bendala J
Nature communications 2025 Jul 3;16(1):6121

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Murine cell lines with defined mutations model different histological subtypes of epithelial ovarian cancer.

Zhang L, Fang Y, Uygun I, Li D, Strange M, Zaidi SK, Wang W, Knight J, Radolec M, Elishaev E, Brozick JF, Edwards A, Tseng G, Cascio S, Buckanovich R, Edwards RP, Vlad AM
Disease models & mechanisms 2025 Jul 1;18(7)

---

RAGE is a key regulator of ductular reaction-mediated fibrosis during cholestasis.

Lam WM, Gabernet G, Poth T, Sator-Schmitt M, Oquendo MB, Kast B, Lohr S, de Ponti A, Weiß L, Schneider M, Helm D, Müller-Decker K, Schirmacher P, Heikenwälder M, Klingmüller U, Schneller D, Geisler F, Nahnsen S, Angel P
EMBO reports 2025 Feb;26(3):880-907

---

Dimethyl fumarate promotes the degradation of HNF1B and suppresses the progression of clear cell renal cell carcinoma.

Dai Y, Li H, Fan S, Wang K, Cui Z, Zhao X, Sun X, Lin M, Li J, Gao Y, Tian Z, Yang H, Zha B, Lv L, Xu Y
Cell death & disease 2025 Feb 6;16(1):71

---

Molecular profiling reveals novel therapeutic targets and clonal evolution in ovarian clear cell carcinoma.

Chao A, Huang CY, Yu W, Lin CY, Lin H, Chao AS, Lin CT, Chou HH, Huang KG, Huang HJ, Chang TC, Rozen SG, Wu RC, Lai CH
BMC cancer 2024 Nov 14;24(1):1403

---

Investigating the efficacy of tissue factor pathway inhibitor‑2 as a promising prognostic marker for ovarian cancer.

Maehana T, Kawaguchi R, Nishikawa K, Kawahara N, Yamada Y, Kimura F
Oncology letters 2024 Jul;28(1):302

---

Small molecules enhance the high-efficiency generation of pancreatic ductal organoids.

Liao Y, Lin Z, Li S, Yin X
Acta biochimica et biophysica Sinica 2024 Dec 5;57(7):1184-1194

---

Clinicopathological correlations of endometrioid and clear cell carcinomas in the uterus and ovary.

Mori H, Nishida H, Kusaba T, Kawamura K, Oyama Y, Daa T
Medicine 2023 Sep 15;102(37):e35301

---

Dnajb11-Kidney Disease Develops from Reduced Polycystin-1 Dosage but not Unfolded Protein Response in Mice.

Ghosh Roy S, Li Z, Guo Z, Tran Long K, Rehrl S, Tian X, Dong K, Besse W
Journal of the American Society of Nephrology : JASN 2023 Sep 1;34(9):1521-1534

---

Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion.

Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K, Cesarano A, Hockemeyer K, Haining WN, Witkowski MT, Qi J, Tsirigos A, Perna F, Aifantis I, Kadoch C
Molecular cell 2023 Apr 20;83(8):1216-1236.e12

---

The Hippo pathway effector TAZ induces intrahepatic cholangiocarcinoma in mice and is ubiquitously activated in the human disease.

Cigliano A, Zhang S, Ribback S, Steinmann S, Sini M, Ament CE, Utpatel K, Song X, Wang J, Pilo MG, Berger F, Wang H, Tao J, Li X, Pes GM, Mancarella S, Giannelli G, Dombrowski F, Evert M, Calvisi DF, Chen X, Evert K
Journal of experimental & clinical cancer research : CR 2022 Jun 3;41(1):192

---

Loss of Arid1a and Pten in Pancreatic Ductal Cells Induces Intraductal Tubulopapillary Neoplasm via the YAP/TAZ Pathway.

Fukunaga Y, Fukuda A, Omatsu M, Namikawa M, Sono M, Masuda T, Araki O, Nagao M, Yoshikawa T, Ogawa S, Hiramatsu Y, Muta Y, Tsuda M, Maruno T, Nakanishi Y, Ferrer J, Tsuruyama T, Masui T, Hatano E, Seno H
Gastroenterology 2022 Aug;163(2):466-480.e6

---

Recombinant protein Schistosoma japonicum-derived molecule attenuates dextran sulfate sodium-induced colitis by inhibiting miRNA-217-5p to alleviate apoptosis.

Zhang LC, Wu XY, Yang RB, Chen F, Liu JH, Hu YY, Wu ZD, Wang LF, Sun X
World journal of gastroenterology 2021 Dec 14;27(46):7982-7994

---

Computational and functional analyses of T2D GWAS SNPs for transcription factor binding.

Cheng M, Huang X, Zhang M, Huang Q
Biochemical and biophysical research communications 2020 Mar 12;523(3):658-665

---

HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.

Wang J, He C, Gao P, Wang S, Lv R, Zhou H, Zhou Q, Zhang K, Sun J, Fan C, Ding G, Lan F
Oncogene 2020 Feb;39(6):1335-1346

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HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.

Lu W, Sun J, Zhou H, Wang F, Zhao C, Li K, Fan C, Ding G, Wang J
Journal of cellular and molecular medicine 2020 Dec;24(24):14539-14548

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Definitive endoderm differentiation is promoted in suspension cultured human iPS-derived spheroids more than in adherent cells.

Yabe SG, Nishida J, Fukuda S, Takeda F, Nashiro K, Ibuki M, Okochi H
The International journal of developmental biology 2019;63(6-7):271-280

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Expression of mutant mRNA and protein in pancreatic cells derived from MODY3- iPS cells.

Yabe SG, Nishida J, Fukuda S, Takeda F, Nasiro K, Yasuda K, Iwasaki N, Okochi H
PloS one 2019;14(5):e0217110

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Tumor-suppressive activity of Hnf1β in Wilms' tumor.

Liu Y, Kanyomse Q, Xie Y
Bioscience, biotechnology, and biochemistry 2019 Nov;83(11):2008-2015

---

The nuclear receptor Shp regulates morphine withdrawal syndrome via modulation of Ugt2b expression in mice.

Chen M, Guo L, Dong D, Yu F, Zhang T, Wu B
Biochemical pharmacology 2019 Mar;161:163-172

---

miR-194 regulates the proliferation and migration via targeting Hnf1β in mouse metanephric mesenchyme cells.

Liu Y, Hu Y, Ni D, Liu J, Xia H, Xu L, Zhou Q, Xie Y
In vitro cellular & developmental biology. Animal 2019 Aug;55(7):512-521

---

Complex bile duct network formation within liver decellularized extracellular matrix hydrogels.

Lewis PL, Su J, Yan M, Meng F, Glaser SS, Alpini GD, Green RM, Sosa-Pineda B, Shah RN
Scientific reports 2018 Aug 15;8(1):12220

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ATM-deficiency increases genomic instability and metastatic potential in a mouse model of pancreatic cancer.

Drosos Y, Escobar D, Chiang MY, Roys K, Valentine V, Valentine MB, Rehg JE, Sahai V, Begley LA, Ye J, Paul L, McKinnon PJ, Sosa-Pineda B
Scientific reports 2017 Sep 11;7(1):11144

---

Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.

Nikolić MZ, Caritg O, Jeng Q, Johnson JA, Sun D, Howell KJ, Brady JL, Laresgoiti U, Allen G, Butler R, Zilbauer M, Giangreco A, Rawlins EL
eLife 2017 Jun 30;6

---

Molecular Mechanism Underlying the Suppression of CPB2 Expression Caused by Persistent Hepatitis C Virus RNA Replication.

Sejima H, Satoh S, Dansako H, Honda M, Kaneko S, Ikeda M, Kato N
Acta medica Okayama 2016;70(2):75-88

---

Hepatocyte Nuclear Factor-1β Induces Redifferentiation of Dedifferentiated Tubular Epithelial Cells.

Omata M, Doke Y, Yamada C, Kawashima K, Sho R, Enomoto K, Furuya M, Inomata N
PloS one 2016;11(5):e0154912

---

Does clear cell carcinoma of stomach exist? Clinicopathological and prognostic significance of clear cell changes in gastric adenocarcinomas.

Kim JY, Park DY, Kim GH, Jeon TY, Lauwers GY
Histopathology 2014 Jul;65(1):90-9

---

Prox1 ablation in hepatic progenitors causes defective hepatocyte specification and increases biliary cell commitment.

Seth A, Ye J, Yu N, Guez F, Bedford DC, Neale GA, Cordi S, Brindle PK, Lemaigre FP, Kaestner KH, Sosa-Pineda B
Development (Cambridge, England) 2014 Feb;141(3):538-47

---

Ovarian cancers arising from endometriosis: a microenvironmental biomarker study including ER, HNF1ß, p53, PTEN, BAF250a, and COX-2.

Lai CR, Hsu CY, Chen YJ, Yen MS, Chao KC, Li AF
Journal of the Chinese Medical Association : JCMA 2013 Nov;76(11):629-34

---

Hepatocyte nuclear factor 1β is a novel prognostic marker independent of the Milan criteria in transplantable hepatocellular carcinoma: a retrospective analysis based on tissue microarrays.

Shim JH, Lee HC, Han S, Kang HJ, Yu E, Lee SG
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2013 Mar;19(3):336-45

---

Clear cell carcinomas of the ovary: a multi-institutional study of 129 cases in Korea with prognostic significance of Emi1 and Galectin-3.

Min KW, Park MH, Hong SR, Lee H, Kwon SY, Hong SH, Joo HJ, Park IA, An HJ, Suh KS, Oh HK, Yoo CW, Kim MJ, Chang HK, Jun SY, Yoon HK, Chang ED, Kim DW, Kim I, Gynecologic Pathology Study Group of the Korean Society of Pathologists
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2013 Jan;32(1):3-14

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 12533-1-AP(HNF1B antibody) at dilution of 1:800

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The HNF1B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human HNF1B. This antibody recognizes human,mouse,rat antigen. The HNF1B antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

12533-1-AP.pdf

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