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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- MA5-24605 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TCF2 Monoclonal Antibody (CL0374)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KEVLVQALEE LLPSPNFGVK LETLPLSPGS GAEPDTKPVF HTLTNGHAKG RLSGDEGSED GDDYDTPPIL KELQALNTEE AAEQRAEVDR MLSEDPWRAA KMIKGYMQQH Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%. Binds to an epitope located within the peptide sequence TPPILKELQA as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL0374
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Utility of HNF-1B and a panel of lineage-specific biomarkers to optimize the diagnosis of pancreatic ductal adenocarcinoma.
Bai S, Lindberg J, Whalen G, Bathini V, Zou J, Yang MX
American journal of cancer research 2021;11(3):858-865
American journal of cancer research 2021;11(3):858-865
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCF2 in Lane1: Marker (kDa), Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: HNF1B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400162). Samples were probed using a TCF2 Monoclonal Antibody (Product # MA5-24605).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TCF2 in human cell lines Caco-2 and A-431 using a TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605). Corresponding HNF1B RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of TCF2 in A549 cell line using TCF2 Monoclonal Antibody (Product # MA5-24605), showing spotty nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human kidney and tonsil tissues using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605). Corresponding HNF1B RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human kidney using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605) shows strong nuclear positivity in cells in tubules.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human colon using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605) shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human renal cancer using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605) shows strong nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human colorectal cancer using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605) shows moderate nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of TCF2 in human tonsil using TCF2 Monoclonal Antibody (CL0374) (Product # MA5-24605) shows no positivity in lymphoid cells as expected.
