Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS4799 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- MARCKS (phospho S163) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 163.
- Description
- Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC Ï« and PKC ÷, but not PKC Ñ´, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-MARCKS (S163) pAb at 1:500 dilutionLane1:MCF-7 cell lysate treated with PMALane2:sp2/0 cell lysate treated with PMALane3:PC12 cell lysate treated with PMA