Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00004082-M06 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004082-M06, RRID:AB_540336
- Product name
- Mouse Monoclonal MARCKS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MARCKS - myristoylated alanine-rich protein kinase C substrate
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of MARCKS expression in Raw 264.7. (The predicted M.W. of MARCKS is 29 to 39 KDa, but it seems the actual M.W. in vivois between 68 to 90 KDa in different species.)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MARCKS Antibody (2C2) [H00004082-M06] - Detection limit for recombinant GST tagged MARCKS is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of monoclonal antibody to MARCKS on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MARCKS Antibody (2C2) [H00004082-M06] - Analysis of monoclonal antibody to MARCKS on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml