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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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- Product number
- PA5-105296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MARCKS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total MARCKS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Protein disulfide isomerase ERp57 protects early muscle denervation in experimental ALS.
Rozas P, Pinto C, Martínez Traub F, Díaz R, Pérez V, Becerra D, Ojeda P, Ojeda J, Wright MT, Mella J, Plate L, Henríquez JP, Hetz C, Medinas DB
Acta neuropathologica communications 2021 Feb 4;9(1):21
Acta neuropathologica communications 2021 Feb 4;9(1):21
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MARCKS in mouse brain tissue lysate (left lane: treated with blocking peptide). Samples were incubated with MARCKS polyclonal antibody (Product # PA5-105296).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MARCKS in mouse brain tissue lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with MARCKS polyclonal antibody (Product # PA5-105296).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MARCKS in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with MARCKS polyclonal antibody (Product # PA5-105296) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MARCKS in A549 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with MARCKS polyclonal antibody (Product # PA5-105296) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
