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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PPS010 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-MARCKS (S152/S156) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Specific for the ~83 kDa MARCKS protein phosphorylated at S152/S156 in Western blots of rat brain homogenates.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Xenopus, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
Submitted references The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
Obis T, Besalduch N, Hurtado E, Nadal L, Santafe MM, Garcia N, Tomàs M, Priego M, Lanuza MA, Tomàs J
Molecular brain 2015 Feb 10;8:8
Molecular brain 2015 Feb 10;8:8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-MARCKS (S152/S156) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda-PPase.
