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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-49610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-2b Adrenergic Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha-2b Adrenergic Receptor in various lysates. Samples were incubated with alpha-2b Adrenergic Receptor polyclonal antibody (Product # PA5-49610) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: MCF-7 whole cell lysate; Lane 2: MDA-MB-231 whole cell lysate; Lane 3: SK-BR-3 whole cell lysate. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ADRA2B in MDA-MB231 cells. Sample was probed with a ADRA2B Antibody (Center) (Product # PA5-49610) followed by Alexa Fluor® 488-conjugated goat rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of alpha-2b Adrenergic Receptor in MDA-MB231 cells. Samples were incubated in alpha-2b Adrenergic Receptor polyclonal antibody (Product # PA5-49610) followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of alpha-2b Adrenergic Receptor in formalin-fixed and paraffin-embedded human breast carcinoma. Samples were incubated with alpha-2b Adrenergic Receptor polyclonal antibody (Product # PA5-49610) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of alpha-2b Adrenergic Receptor in formalin-fixed and paraffin-embedded human breast carcinoma. Samples were incubated with alpha-2b Adrenergic Receptor polyclonal antibody (Product # PA5-49610) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ADRA2B in MDA-231 cells using ADRA2B Antibody (Center) (Product # PA5-49610) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of alpha-2b Adrenergic Receptor in MDA-231 cells (bottom histogram). Samples were incubated with alpha-2b Adrenergic Receptor polyclonal antibody (Product # PA5-49610) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control (top histogram).
