Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-49610 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-2b Adrenergic Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADRA2B in MDA-MB231 cell line lysates (35 µg/lane). ADRA2B (arrow) was detected using a ADRA2B Antibody (Center) (Product # PA5-49610).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ADRA2B in MDA-MB231 cells. Sample was probed with a ADRA2B Antibody (Center) (Product # PA5-49610) followed by Alexa Fluor® 488-conjugated goat rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADRA2B in Formalin-fixed and paraffin-embedded human breast carcinoma with tissue. Samples were probed with a ADRA2B Antibody (Center) (Product # PA5-49610) followed by peroxidase-conjugation to the secondary antibody, and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ADRA2B in MDA-231 cells using ADRA2B Antibody (Center) (Product # PA5-49610) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat anti-rabbit secondary antibodies were used for the analysis.