F54979

antibody from NSJ Bioreagents

Targeting: ADRA2B ADRA2L1, ADRA2RL1, ADRARL1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54979

Provider

NSJ Bioreagents

Product name

Alpha-2B Adrenergic Receptor Antibody / ADRA2B

Antibody type

Polyclonal

Description

This highly specific Alpha-2B Adrenergic Receptor antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Alpha-2B Adrenergic Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) MCF7, 2) MDA-MB-231 and 3) SK-BR-3 cell lysate with Alpha-2B Adrenergic Receptor antibody. Predicted molecular weight ~50 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with Alpha-2B Adrenergic Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-231 cells with Alpha-2B Adrenergic Receptor antibody; Blue=isotype control, Green= Alpha-2B Adrenergic Receptor antibody.