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LearnH00002969-M01
antibody from Abnova Corporation
Targeting: GTF2I
BAP-135, BTKAP1, DIWS, IB291, SPIN, TFII-I, WBSCR6
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00002969-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00002969-M01, RRID:AB_464329
- Product name
- GTF2I monoclonal antibody (M01), clone 3E2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant GTF2I.
- Antigen sequence
ELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK
DFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVD
AVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK
MLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILE
NKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILS
PGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESED
PDYYQYNIQGSHHSSEGNEGTEMEVPAEG- Isotype
- IgG
- Antibody clone number
- 3E2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references An SNP in an ultraconserved regulatory element affects Dlx5/Dlx6 regulation in the forebrain.
Poitras L, Yu M, Lesage-Pelletier C, Macdonald RB, Gagné JP, Hatch G, Kelly I, Hamilton SP, Rubenstein JL, Poirier GG, Ekker M
Development (Cambridge, England) 2010 Sep;137(18):3089-97
Development (Cambridge, England) 2010 Sep;137(18):3089-97
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GTF2I monoclonal antibody (M01), clone 3E2. Western Blot analysis of GTF2I expression in human colon.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged GTF2I is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to GTF2I on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to GTF2I on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
