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LearnF54433
antibody from NSJ Bioreagents
Targeting: GTF2I
BAP-135, BTKAP1, DIWS, IB291, SPIN, TFII-I, WBSCR6
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F54433 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GTF2I Antibody / TFII-I
- Antibody type
- Polyclonal
- Description
- This highly specific GTF2I antibody is suitable for use in Immunofluorescence/Western blot/Flow cytometry/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the GTF2I antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431 and 2) HeLa lysate with GTF2I antibody. Predicted molecular weight ~112 kDa but can be observed at up to ~135 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human A549 cells with GTF2I antibody (green) and anti-Actin (red).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HeLa cells with GTF2I antibody (green) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with GTF2I antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with GTF2I antibody; Blue=isotype control, Green= GTF2I antibody.
