Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 12167-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12167-1-AP, RRID:AB_2286502
- Product name
- SMAD5 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated SMAD5 antibody (Cat. #12167-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.
Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.
A negative feedback loop centered on SMAD3 expression in transforming growth factor β1-induced corneal myofibroblast differentiation.
Efficacy of Danggui Buxue decoction on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism.
Discovery of Highly Potent and BMPR2-Selective Kinase Inhibitors Using DNA-Encoded Chemical Library Screening.
A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis.
Integrative Analysis of ceRNA Networks in human periodontal ligament stem cells under hypoxia.
BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.
CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway.
Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells.
Splicing factor SRSF6 mediates pleural fibrosis.
Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling.
Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.
Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.
BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.
SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.
Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.
The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain.
Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.
Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, Monsivais D, Matzuk M
eLife 2024 Mar 27;12
eLife 2024 Mar 27;12
Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.
Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, Monsivais D, Matzuk MM
bioRxiv : the preprint server for biology 2024 Feb 7;
bioRxiv : the preprint server for biology 2024 Feb 7;
A negative feedback loop centered on SMAD3 expression in transforming growth factor β1-induced corneal myofibroblast differentiation.
Tang Y, Du E, Wang G, Qin F, Meng Z, Dai L, Wang Y, Ren S
Experimental eye research 2023 Nov;236:109654
Experimental eye research 2023 Nov;236:109654
Efficacy of Danggui Buxue decoction on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism.
Ye Z, Xian W, Ling GU, Zihang LI, Jingtian Z, Wenkai W, Liang Z, Mei X
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2023 Jun;43(3):507-513
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2023 Jun;43(3):507-513
Discovery of Highly Potent and BMPR2-Selective Kinase Inhibitors Using DNA-Encoded Chemical Library Screening.
Modukuri RK, Monsivais D, Li F, Palaniappan M, Bohren KM, Tan Z, Ku AF, Wang Y, Madasu C, Li JY, Tang S, Miklossy G, Palmer SS, Young DW, Matzuk MM
Journal of medicinal chemistry 2023 Feb 9;66(3):2143-2160
Journal of medicinal chemistry 2023 Feb 9;66(3):2143-2160
A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis.
Zhang L, Fang Z, Cheng G, He M, Lin Y
Cell death & disease 2023 Feb 20;14(2):145
Cell death & disease 2023 Feb 20;14(2):145
Integrative Analysis of ceRNA Networks in human periodontal ligament stem cells under hypoxia.
Ye Y, Fu L, Liu L, Xiao T, Cuba Manduca AG, Yu J
Oral diseases 2023 Apr;29(3):1197-1213
Oral diseases 2023 Apr;29(3):1197-1213
BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.
Peng W, Zhou X, Xu T, Mao Y, Zhang X, Liu H, Liang L, Liu L, Liu L, Xiao Y, Zhang F, Li S, Shi M, Zhou Y, Tang L, Wang Y, Guo B
Cell death & disease 2022 Mar 21;13(3):254
Cell death & disease 2022 Mar 21;13(3):254
CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway.
Ye Y, Ke Y, Liu L, Xiao T, Yu J
Stem cells international 2021;2021:5177488
Stem cells international 2021;2021:5177488
Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells.
Ma X, Xiang F, Pei Z, Miao J, Wu P, Song X, Li Y, Zhang Y
Genes & diseases 2021 May;8(3):364-372
Genes & diseases 2021 May;8(3):364-372
Splicing factor SRSF6 mediates pleural fibrosis.
Liang LM, Xiong L, Cheng PP, Chen SJ, Feng X, Zhou YY, Niu Q, Wang M, Chen Q, Song LJ, Yu F, He XL, Xiang F, Wang X, Ye H, Ma WL
JCI insight 2021 May 24;6(10)
JCI insight 2021 May 24;6(10)
Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling.
Yin P, Li D, Zhao Q, Cai M, Wu Z, Shi Y, Su L
Cell death discovery 2021 Dec 14;7(1):391
Cell death discovery 2021 Dec 14;7(1):391
Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.
Wang X, Liao X, Zhang Y, Wei L, Pang Y
Scientific reports 2020 Sep 2;10(1):14476
Scientific reports 2020 Sep 2;10(1):14476
Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.
Ni C, Fang QQ, Chen WZ, Jiang JX, Jiang Z, Ye J, Zhang T, Yang L, Meng FB, Xia WJ, Zhong M, Huang J
Signal transduction and targeted therapy 2020 Apr 29;5(1):41
Signal transduction and targeted therapy 2020 Apr 29;5(1):41
BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.
Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, Cuellar Camacho JL, Haag R, Ruppert C, Sengle G, Cavalcanti-Adam EA, Blank KG, Knaus P
PLoS biology 2019 Dec;17(12):e3000557
PLoS biology 2019 Dec;17(12):e3000557
SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.
Chen J, Li C, Zhan R, Yin Y
Biochemical and biophysical research communications 2018 Jun 18;501(1):220-225
Biochemical and biophysical research communications 2018 Jun 18;501(1):220-225
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
Zhu L, Ma J, Mu R, Zhu R, Chen F, Wei X, Shi X, Zang S, Jin L
Life sciences 2018 Jun 1;202:175-181
Life sciences 2018 Jun 1;202:175-181
IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.
Dörpholz G, Murgai A, Jatzlau J, Horbelt D, Belverdi MP, Heroven C, Schreiber I, Wendel G, Ruschke K, Stricker S, Knaus P
Scientific reports 2017 Aug 18;7(1):8778
Scientific reports 2017 Aug 18;7(1):8778
Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.
Zeng Y, Liu H, Kang K, Wang Z, Hui G, Zhang X, Zhong J, Peng W, Ramchandran R, Raj JU, Gou D
Scientific reports 2015 Jul 13;5:12098
Scientific reports 2015 Jul 13;5:12098
The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain.
Yao QP, Zhang P, Qi YX, Chen SG, Shen BR, Han Y, Yan ZQ, Jiang ZL
The international journal of biochemistry & cell biology 2014 Apr;49:98-104
The international journal of biochemistry & cell biology 2014 Apr;49:98-104
Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.
Zhang XY, Shen BR, Zhang YC, Wan XJ, Yao QP, Wu GL, Wang JY, Chen SG, Yan ZQ, Jiang ZL
PloS one 2013;8(3):e59002
PloS one 2013;8(3):e59002
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP( SMAD5 Antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line