12167-1-AP

antibody from Proteintech Group

Targeting: SMAD5 Dwfc, JV5-1, MADH5

Provider product page for 12167-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [31]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

Submit

Product number: 12167-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12167-1-AP, RRID:AB_2286502
Product name: SMAD5 antibody
Antibody type: Polyclonal
Description: KD/KO validated SMAD5 antibody (Cat. #12167-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

SMAD5 protein structure - 12167-1-AP shown in red.

Submitted references Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair.

Lin F, Yao L, Xu Y, Xiang L, Zhang N, Zhuang Y, Jiang Q, Zhang Z, Cao Q, Chen J, Jiang L, Qian S, Shi Y, Xue D, Bao L, Yu X, Ye Z, Cui W
Bioactive materials 2026 Sep;63:795-817

SENP6-mediated desumoylation of SMAD5 regulates osteogenic fate in periodontal ligament stem cells.

Jin H, Liu C, Wang W, Xie H, Ji B, Wei W, Wang X
European journal of medical research 2026 Jan 30;31(1)

Paneth-like transition drives resistance to dual targeting of KRAS and EGFR in colorectal cancer.

Zhang Y, Chen J, She Y, Fang Z, Zhang Y, Ruan D, Guo W, Liao J, Zhou W, Lao J, Fang W, Pan X, Kang W, Wang Z, Wu Y, Deng R, Tian L, Wang L, Huang H, Zheng J, Yan Y, Lu H, Wang R, Yaeger R, Zhao Q, Liao W, Wang F, Gao Y
Cancer cell 2026 Jan 12;44(1):77-93.e8

KDM4A-driven SMAD5/TGFβ axis activation as a therapeutic target in cholangiocarcinoma: mechanistic insights and translational implications.

Wang Q, Kuai Y, Xiong F, Wang D, Wu G, Liu W, Chen J, Wang B, Chen Y
Cell & bioscience 2025 Sep 29;15(1):128

Qiang Jin Mixture Promotes Osteogenic Differentiation of MC3T3-E1 Cells via BMP2/Smads Pathway and its Network Pharmacology Study.

Gong W, Zhu Y, Wang L
Molecular biotechnology 2025 Nov;67(11):4150-4166

MicroRNA-93-5p alleviates uric acid-induced fibrosis in renal tubular epithelial cells by regulating the SMAD5/Id2 signaling pathway.

Lu X, Ma C, Li D, Yang J, Sun Y, Liu B, Zhao B, Li H
Cellular signalling 2025 Nov;135:112081

Near-infrared responsive nanozyme-carboxymethyl chitosan/chondroitin sulfate composite hydrogel: Multicomponent synergy optimizes bone defect microenvironment to enhance repair.

Sun D, Ding C, Wang N, Ding Q, Liu W, Liu X, Wei H, Yang J, Cheng Z, Zhao S, Hong B, Zhao T
International journal of biological macromolecules 2025 Dec;332(Pt 1):148646

Metabolic Interplay in Acute Lung Injury: PARK7 Integrates FADS1/2-Dependent PUFA Metabolism and H3K14 Lactylation to Attenuate Endothelial Ferroptosis and Dysfunction.

Xu J, Wang Y, Mao W, Wei T, Li Y, Song J, Zhang C, Chen X, Chen C, Xu Q, Wu X, Song Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Dec;12(46):e08725

LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis.

Hao S, Zuo F, Zhang H, Wang Y, Huang L, Ma F, Song T, Zhang T, Ren X, Wang N
IUBMB life 2024 Sep;76(9):731-744

Strategies for arterial graft optimization at the single-cell level.

Hu Z, Dai M, Chang Y, Hua X, Zhang N, Chen X, Sheng Y, Xu Z, Zhang H, Zhang Y, Cui H, Jia H, Wang XJ, Song J
Nature cardiovascular research 2024 May;3(5):541-557

Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.

Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, Monsivais D, Matzuk M
eLife 2024 Mar 27;12

Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy.

Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, Monsivais D, Matzuk MM
bioRxiv : the preprint server for biology 2024 Feb 7;

A negative feedback loop centered on SMAD3 expression in transforming growth factor β1-induced corneal myofibroblast differentiation.

Tang Y, Du E, Wang G, Qin F, Meng Z, Dai L, Wang Y, Ren S
Experimental eye research 2023 Nov;236:109654

Efficacy of Danggui Buxue decoction on diabetic nephropathy-induced renal fibrosis in rats and possible mechanism.

Ye Z, Xian W, Ling GU, Zihang LI, Jingtian Z, Wenkai W, Liang Z, Mei X
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2023 Jun;43(3):507-513

Discovery of Highly Potent and BMPR2-Selective Kinase Inhibitors Using DNA-Encoded Chemical Library Screening.

Modukuri RK, Monsivais D, Li F, Palaniappan M, Bohren KM, Tan Z, Ku AF, Wang Y, Madasu C, Li JY, Tang S, Miklossy G, Palmer SS, Young DW, Matzuk MM
Journal of medicinal chemistry 2023 Feb 9;66(3):2143-2160

A novel Hoxd13 mutation causes synpolydactyly and promotes osteoclast differentiation by regulating pSmad5/p65/c-Fos/Rank axis.

Zhang L, Fang Z, Cheng G, He M, Lin Y
Cell death & disease 2023 Feb 20;14(2):145

Integrative Analysis of ceRNA Networks in human periodontal ligament stem cells under hypoxia.

Ye Y, Fu L, Liu L, Xiao T, Cuba Manduca AG, Yu J
Oral diseases 2023 Apr;29(3):1197-1213

BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.

Peng W, Zhou X, Xu T, Mao Y, Zhang X, Liu H, Liang L, Liu L, Liu L, Xiao Y, Zhang F, Li S, Shi M, Zhou Y, Tang L, Wang Y, Guo B
Cell death & disease 2022 Mar 21;13(3):254

CircRNA FAT1 Regulates Osteoblastic Differentiation of Periodontal Ligament Stem Cells via miR-4781-3p/SMAD5 Pathway.

Ye Y, Ke Y, Liu L, Xiao T, Yu J
Stem cells international 2021;2021:5177488

Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells.

Ma X, Xiang F, Pei Z, Miao J, Wu P, Song X, Li Y, Zhang Y
Genes & diseases 2021 May;8(3):364-372

Splicing factor SRSF6 mediates pleural fibrosis.

Liang LM, Xiong L, Cheng PP, Chen SJ, Feng X, Zhou YY, Niu Q, Wang M, Chen Q, Song LJ, Yu F, He XL, Xiang F, Wang X, Ye H, Ma WL
JCI insight 2021 May 24;6(10)

Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling.

Yin P, Li D, Zhao Q, Cai M, Wu Z, Shi Y, Su L
Cell death discovery 2021 Dec 14;7(1):391

Schisandrin B regulates MC3T3-E1 subclone 14 cells proliferation and differentiation through BMP2-SMADs-RUNX2-SP7 signaling axis.

Wang X, Liao X, Zhang Y, Wei L, Pang Y
Scientific reports 2020 Sep 2;10(1):14476

Breast cancer-derived exosomes transmit lncRNA SNHG16 to induce CD73+γδ1 Treg cells.

Ni C, Fang QQ, Chen WZ, Jiang JX, Jiang Z, Ye J, Zhang T, Yang L, Meng FB, Xia WJ, Zhong M, Huang J
Signal transduction and targeted therapy 2020 Apr 29;5(1):41

BMPR2 acts as a gatekeeper to protect endothelial cells from increased TGFβ responses and altered cell mechanics.

Hiepen C, Jatzlau J, Hildebrandt S, Kampfrath B, Goktas M, Murgai A, Cuellar Camacho JL, Haag R, Ruppert C, Sengle G, Cavalcanti-Adam EA, Blank KG, Knaus P
PLoS biology 2019 Dec;17(12):e3000557

SPG6 supports development of acute myeloid leukemia by regulating BMPR2-Smad-Bcl-2/Bcl-xl signaling.

Chen J, Li C, Zhan R, Yin Y
Biochemical and biophysical research communications 2018 Jun 18;501(1):220-225

Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.

Zhu L, Ma J, Mu R, Zhu R, Chen F, Wei X, Shi X, Zang S, Jin L
Life sciences 2018 Jun 1;202:175-181

IRS4, a novel modulator of BMP/Smad and Akt signalling during early muscle differentiation.

Dörpholz G, Murgai A, Jatzlau J, Horbelt D, Belverdi MP, Heroven C, Schreiber I, Wendel G, Ruschke K, Stricker S, Knaus P
Scientific reports 2017 Aug 18;7(1):8778

Hypoxia inducible factor-1 mediates expression of miR-322: potential role in proliferation and migration of pulmonary arterial smooth muscle cells.

Zeng Y, Liu H, Kang K, Wang Z, Hui G, Zhang X, Zhong J, Peng W, Ramchandran R, Raj JU, Gou D
Scientific reports 2015 Jul 13;5:12098

The role of SIRT6 in the differentiation of vascular smooth muscle cells in response to cyclic strain.

Yao QP, Zhang P, Qi YX, Chen SG, Shen BR, Han Y, Yan ZQ, Jiang ZL
The international journal of biochemistry & cell biology 2014 Apr;49:98-104

Induction of thoracic aortic remodeling by endothelial-specific deletion of microRNA-21 in mice.

Zhang XY, Shen BR, Zhang YC, Wan XJ, Yao QP, Wu GL, Wang JY, Chen SG, Yan ZQ, Jiang ZL
PloS one 2013;8(3):e59002

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 12167-1-AP( SMAD5 Antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using SMAD5 antibody 12167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line