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- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF2250 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Smad5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Smad5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Smad1, rhSmad2 and rhSmad4 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Targeting of SMAD5 links microRNA-155 to the TGF-beta pathway and lymphomagenesis.
Rai D, Kim SW, McKeller MR, Dahia PL, Aguiar RC
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3111-6
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3111-6
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Smad5 in Human Colon. Smad5 was detected in immersion fixed paraffin-embedded sections of human colon using 5 µg/mL Human Smad5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2250) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
