AM06562SU-N

antibody from OriGene

Targeting: SMAD5 Dwfc, JV5-1, MADH5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM06562SU-N

Provider

OriGene

Product name

SMAD5 mouse monoclonal antibody, clone 3H9, Ascites

Antibody type

Monoclonal

Description

SMAD5 mouse monoclonal antibody, clone 3H9, Ascites

Host

Mouse

Conjugate

Unconjugated

Epitope

SMAD5

Isotype

IgG

Antibody clone number

3H9

Vial size

100 µl

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of NTERA-2 cells using SMAD5 antibody Cat.-No AM06562SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells using SMAD5 antibody Cat.-No AM06562SU-N (green) and negative control (purple).

Validation comment

FC