H00004090-M05

antibody from Abnova Corporation

Targeting: SMAD5 Dwfc, JV5-1, MADH5

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004090-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004090-M05, RRID:AB_894263

Product name

SMAD5 monoclonal antibody (M05), clone 4F10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SMAD5.

Antigen sequence

KPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPV
LVPRHNEFNPQHSLLVQFRNLSHNEPHMPQNATFP
DSFHQPNN


Isotype

IgG

Antibody clone number

4F10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SMAD5 monoclonal antibody (M05), clone 4F10 Western Blot analysis of SMAD5 expression in HeLa ( Cat # L013V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SMAD5 is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol