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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10322, RRID:AB_11189528
- Product name
- SMAD5 monoclonal antibody, clone 3H9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant SMAD5.
- Isotype
- IgG
- Antibody clone number
- 3H9
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) against HeLa (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K-562 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NTERA-2 cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissue (A) and lung cancer tissue (B) using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using SMAD5 monoclonal antobody, clone 3H9 (Cat # MAB10322) (green) and negative control (purple).
- Validation comment
- Flow Cytometry
