P01423-1

antibody from Boster Biological Technology

Targeting: SMAD5 Dwfc, JV5-1, MADH5

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

P01423-1

Provider

Boster Biological Technology

Product name

Anti-Phospho-Smad5 (S463/465) Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Phospho-Smad5 (S463/465) detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AFB-19

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Pilose antler extract promotes hair growth in androgenic alopecia mice by promoting the initial anagen phase.

Liu M, He G, Wang F, Sun Y, Ma S, Hao Y, Wang Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2024 May;174:116503

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody(P01423-1)SMAD5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SMAD5 Antibody (P01423-1)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image