Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM06562SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM06562SU-N, RRID:AB_11218152
- Product name
- anti SMAD5
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant fragment of Human SMAD5 expressed in E. Coli.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H9
- Vial size
- 0.1 ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using SMAD5 antibody Cat.-No AM06562SU-N against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NTERA-2 cells using SMAD5 antibody Cat.-No AM06562SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 antibody Cat.-No AM06562SU-N with DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using SMAD5 antibody Cat.-No AM06562SU-N (green) and negative control (purple).