Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 44-830 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-SHC (Tyr239, Tyr240) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Input-output behavior of ErbB signaling pathways as revealed by a mass action model trained against dynamic data.
Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.
Insulin enhances growth hormone induction of the MEK/ERK signaling pathway.
Chen WW, Schoeberl B, Jasper PJ, Niepel M, Nielsen UB, Lauffenburger DA, Sorger PK
Molecular systems biology 2009;5:239
Molecular systems biology 2009;5:239
Paxillin-Y118 phosphorylation contributes to the control of Src-induced anchorage-independent growth by FAK and adhesion.
Sachdev S, Bu Y, Gelman IH
BMC cancer 2009 Jan 12;9:12
BMC cancer 2009 Jan 12;9:12
Insulin enhances growth hormone induction of the MEK/ERK signaling pathway.
Xu J, Keeton AB, Franklin JL, Li X, Venable DY, Frank SJ, Messina JL
The Journal of biological chemistry 2006 Jan 13;281(2):982-92
The Journal of biological chemistry 2006 Jan 13;281(2):982-92
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 FAK and adhesion modulate v-Src-induced phosphorylation of various Src substrates . (A) Lysates from adherent cultures of FAK+/+[puro], FAK-/-[puro], FAK+/+[v-Src] and FAK-/-[v-Src] cells were probed either directly by IB for specific phosphorylated form(s) of the Src substrate proteins, total substrate protein levels or GAPDH, or probed for total phosphorylated protein by immunoprecipitating with substrate-specific Ab followed by IB for phosphotyrosine using MAb4G10. (B) Same IB or IP/IB analysis as in panel A using lysates of suspension cultures. Each of these blots is typical of at least duplicate independent experiments.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Phosphorylation of paxillin poY118 is required for enhanced AIG by FAK-/-[v-Src] cells . ( A ) Left panel- IB analysis of FAK+/+[v-Src] or FAK-/-[v-Src] cell clones (""cl."") stably transfected with empty vector (--) or a GFP-paxillin Y118F -expressing vector, probed with Abs specific for GFP, paxillin poY118 or GAPDH. Aliquots of these cells were analyzed by anchorage-independent growth (top right) or for clonogenic efficiency (bottom right) as described in Materials and Methods. Error bars, S.E. *, P < 0.01. ( B ) A similar analysis as in panel A except on cells stably expressing FLAG-p120 Y228F , with IBs probed for FLAG, p120catenin poY228 or GAPDH. Note that there is no statistical difference in the p120catenin Y228F -mediated decrease in AIG between the FAK+/+[v-Src] and FAK-/-[v-Src] cells. ( C ) A similar analysis as in panel A except on cells stably expressing GST-Shc Y239/240F , with IBs probed for GST-tag, Shc poY239/240 , or GAPDH.
