TA323832

antibody from OriGene

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA323832

Provider

OriGene

Product name

Anti-SHC1 (Phospho-Tyr349) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-SHC1 (Phospho-Tyr349) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

SHC1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 63 kDa . Positive control: HepG2 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HepG2 cells untreated with EGF lysate Lane2: HepG2 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using SHC1 (Phospho-Tyr349) antibody at dilution 1/100. (Original magnification:?—200)

Validation comment

IF