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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- R30997 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SHC Antibody / SHC1 / SHC transforming protein 1
- Antibody type
- Polyclonal
- Description
- This highly specific SHC antibody is suitable for use in Western blot/Immunohistochemistry/Immunohistochemistry/Immunocytochemistry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the SHC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SHC antibody and Lane 1: rat brain; and human samples 2: A549; 3: A431; 4: 293T; 5: HeLa; 6: Jurkat cell lysate. Predicted size ~63KD
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SHC antibody and Lane 1: Recombinant human protein 10ng; 2: 5ng; 3: 2.5ng
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of SHC antibody and A549 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: SHC1 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: SHC antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of SHC antibody and rat brain tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-F testing of SHC antibody and rat intestine tissue
