AM00140PU-N

antibody from OriGene

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM00140PU-N

Provider

OriGene

Product name

SHC (SHC1) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 11F6, Purified

Antibody type

Monoclonal

Description

SHC (SHC1) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 11F6, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

SHC1

Isotype

IgG

Antibody clone number

11F6

Vial size

100 µg

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc-11F6 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3

Validation comment

WB