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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00142PU-N - Provider product page
- Provider
- OriGene
- Product name
- SHC (SHC1) pTyr317 (incl. pos. control) mouse monoclonal antibody, clone 15E11, Purified
- Antibody type
- Monoclonal
- Description
- SHC (SHC1) pTyr317 (incl. pos. control) mouse monoclonal antibody, clone 15E11, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SHC1
- Isotype
- IgG
- Antibody clone number
- 15E11
- Vial size
- 100 µg
- Concentration
- NULL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or pervanadate treated (VH) A549 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-15E11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).
- Validation comment
- WB
