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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AM00143PU-N - Provider product page
- Provider
- OriGene
- Product name
- SHC (SHC1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 24E4, Purified
- Antibody type
- Monoclonal
- Description
- SHC (SHC1) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 24E4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SHC1
- Isotype
- IgG
- Antibody clone number
- 24E4
- Vial size
- 100 µg
- Concentration
- 0.1 mg/ml
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3
- Validation comment
- WB
