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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- AM20940PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20940PU-N, RRID:AB_10844451
- Product name
- anti SHC1 / SHC
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein, SHC1 (AAH33925, 171 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 3F4
- Vial size
- 50 µg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SHC1 monoclonal antibody clone 3F4 Western Blot analysis of SHC1 expression in A-431.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SHC1 is approximately 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to SHC1 on A-431 cell (antibody concentration 10 µg/ml).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1/1200 and anti-SHC1 mouse monoclonal antibody 1/50.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Colon, myenteric plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)
