AM00141PU-N

antibody from Acris Antibodies GmbH

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00141PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00141PU-N, RRID:AB_1007316

Product name

anti SHC1 / SHC pTyr239/240 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopeptide conjugated to KLH

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

1E3

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Phosphospecificity Whole cell extracts of control (co), EGF-stimulated (EGF) or pervanadate-treated (VH) A549 tumor cells were applied to SDS-PAGE (ca. 20.000 cells per lane) and transferred to a PVDF membrane. The immunoblot was probed with mab shc-1E3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec).