AM00143PU-N

antibody from Acris Antibodies GmbH

Targeting: SHC1 p66, SHC, ShcA

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00143PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00143PU-N, RRID:AB_1007314

Product name

anti SHC1 / SHC (N-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to KLH. Epitope: N-terminus.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

24E4

Vial size

0.1 mg

Concentration

0.1 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous p66shc: Whole cell lysates of serum starved tumor cells (ca. 20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab shc/p66-24E4 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLaLane 2: HepG2Lane 3: HEK293Lane 4: SH-SY5YLane 5: MDCKLane 6: PC12Lane 7: CMT 93Lane 8: Neuro 2ALane 9: 3T3