MA5-16147

DUX4 antibody from Invitrogen Antibodies

Western blot

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ELISA

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Immunocytochemistry

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Immunohistochemistry

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Other assay

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Provider product page for MA5-16147

Antibody Data

Product number

MA5-16147

Provider

Invitrogen Antibodies

Product name

DUX4 Monoclonal Antibody (P4H2)

Provider product page

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

P4H2

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Superficial sarcomas with CIC rearrangement are aggressive neoplasms: A series of eight cases.
Ko JS, Marusic Z, Azzato EM, Farkas DH, Van Arnam J, Seiwerth S, Fritchie K, Patel RM, Rubin BP, Billings SD
Journal of cutaneous pathology 2020 Jun;47(6):509-516

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DUX4 Immunohistochemistry Is a Highly Sensitive and Specific Marker for CIC-DUX4 Fusion-positive Round Cell Tumor.
Siegele B, Roberts J, Black JO, Rudzinski E, Vargas SO, Galambos C
The American journal of surgical pathology 2017 Mar;41(3):423-429

Western blot

Supportive validation

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Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUX4 using a monoclonal antibody (Product # MA5-16147).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUX4 in total protein from human testis tissue and human K562, HeLa and HepG2 cell lines. Samples were incubated in DUX4 monoclonal antibody (Product # MA5-16147) using a dilution of 2.0 µg/mL followed by an anti-mouse HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUX4 in (1) human testis and (2) DUX4 protein lysates. Samples were incubated in DUX4 monoclonal antibody (Product # MA5-16147).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DUX4 in 0.5 mg/mL HeLa lysate. Samples were incubated in DUX4 monoclonal (Product # MA5-16147). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DUX4 using a monoclonal antibody (Product # MA5-16147).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DUX4 in C2C12 mouse myoblasts transfected with pCS2-DUX4. Samples were incubated in DUX4 monoclonal antibody (Product # MA5-16147). Cells were counterstained with DAPI for nuclei.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DUX4 in human testis. Samples were incubated in DUX4 monoclonal antibody (Product # MA5-16147).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A, Case 6. Occasional microcystic and corded architecture (hematoxylin and eosin [H&E], x200) noted. B and C. Case 3, Foci of rhabdoid and plasmacytoid features (H&E, x40 and x200). D, A representative image of DUX4 immunohistochemical staining is from case 7 (x200)