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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6014 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TXNIP Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific TXNIP antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the TXNIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat brain, 2) rat kidney, 3) rat PC-13, 4) mouse brain, 5) mouse kidney, 6) mouse NIH 3T3, 7) mouse RAW264.7 and human 8) K562, 9) HeLa, 10) HEK293, 11) HL-60, 12) A549, 13) Caco-2 and 14) Raji lysate with TXNIP antibody. Predicted molecular weight ~46 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human ovarian cancer with TXNIP antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human ThP-1 cells with TXNIP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TXNIP antibody.
