PA5-23485
antibody from Invitrogen Antibodies
Targeting: TXNIP
ARRDC6, EST01027, HHCPA78, THIF, VDUP1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-23485 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TXNIP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TxNIP in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide using a TxNIP polyclonal antibody (Product # PA5-23485) at 2 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TXNIP in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in TXNIP polyclonal antibody (Product # PA5-23485) followed by a goat anti-rabbit IgG HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TXNIP Polyclonal Antibody (Product # PA5-23485) and a 43 kDa band corresponding to TXNIP was observed across all cell lines and tissue and increased upon treatment with H2O2 and IGF1 in HEK-293 while decreased upon H2O2 treatment in HeLa. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HEK-293 treated with H2O2 (300mM for 2hr) (Lane 2), HEK-293 treated with IGF1 (50ng/ml for 2hr) (Lane 3), HeLa (Lane 4), HeLa treated with H2O2 (300mM for 2hr) (Lane 5) and Mouse brain (Lane 6) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).