Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB7894 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Syntaxin 4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Syntaxin 4 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q12846
- Isotype
- IgG
- Antibody clone number
- 884637
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Syntaxin 4 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Syntaxin 4 Monoclonal Antibody (Catalog # MAB7894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Syntaxin 4 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Syntaxin 4 by Simple WesternTM. Simple Western lane view shows lysates of SH-SY5Y human neuroblastoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Syntaxin 4 at approximately 42 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human Syntaxin 4 Monoclonal Antibody (Catalog # MAB7894) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syntaxin 4 in U-87 MG Human Cell Line. Syntaxin 4 was detected in immersion fixed U-87 MG human glioblastoma/astrocytoma cell line using Mouse Anti-Human Syntaxin 4 Monoclonal Antibody (Catalog # MAB7894) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and focal contacts. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.