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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00006810-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006810-M04, RRID:AB_566217
- Product name
- STX4A monoclonal antibody (M04), clone 6D1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant STX4A.
- Antigen sequence
DKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIV
KLGNKVQELEKQQVTILATPLPEESMKQELQNLRD
EIKQLGREIRLQLKAIEPQKEEADENYNSVN- Isotype
- IgG
- Antibody clone number
- 6D1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- STX4A monoclonal antibody (M04), clone 6D1. Western Blot analysis of STX4A expression in Raw 264.7(Cat # L024V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of STX4 expression in transfected 293T cell line by STX4A monoclonal antibody (M04), clone 6D1.Lane 1: STX4 transfected lysate (Predicted MW: 34.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged STX4A is approximately 0.1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to STX4A on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
