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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-51560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaxin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MRDRTHELRQ GDDSSDEEDK ERVALVVHPG TARLGSPDEE FFHKVRTIRQ TIVKLGNKVQ ELEKQQVTIL ATPLPEESMK QELQNLRDEI KQLGREIRLQ LKAIEPQKEE ADENYNSVNT RMRKTQHGVL SQQFVELINK CNSMQSE
- Concentration
- 0.3 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Syntaxin 4 in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a Syntaxin 4 Polyclonal Antibody (Product # PA5-51560). Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Syntaxin 4 in human cell line MCF-7 using a Syntaxin 4 Polyclonal Antibody (Product # PA5-51560).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Syntaxin-4 was performed by loading 60 µg of WT (lane 1) and STX4 CRISPR KO (lane 2) HAP1 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. STX4 was detected at approximately 34 kDa (designated by the black arrow) using a STX4 polyclonal antibody (Product # PA5-51560) at a dilution of 1:1,000 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 65-6120) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of STX4 in human cell line U-251 MG shows positivity in plasma membrane. Samples were probed using a STX4 Polyclonal Antibody (Product # PA5-51560).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of STX4 in human skin using a STX4 Polyclonal Antibody (Product # PA5-51560) shows membranous positivity in epidermal cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Syntaxin-4 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of STX4 polyclonal antibody (Product # PA5-51560) with 30 µL of protein A-Sepharose beads and rocked overnight at 4°C. 500 µg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4°C. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using the same antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
