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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-38156 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syntaxin 4 Recombinant Rabbit Monoclonal Antibody (ARC2113)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: A-549, Mouse lung, Rat brain Immunogen sequence: QTIVKLGNKV QELEKQQVTI LATPLPEESM KQELQNLRDE IKQLGREIRL QLKAIEPQKE EADENYNSVN TRMRKTQHGV LSQQFVELIN KCNSMQSEYR E
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- ARC2113
- Vial size
- 100 µL
- Concentration
- 1.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Syntaxin 4 in NIH-3T3 cells using Syntaxin 4 Recombinant Monoclonal Antibody (Product # MA5-38156) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Syntaxin-4 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of STX4 recombinant monoclonal antibody (Product # MA5-38156) with 30 µL of protein A-Sepharose beads and rocked overnight at 4 deg. 500 µg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4 deg. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using the same antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
