MA5-38156

antibody from Invitrogen Antibodies

Targeting: STX4 p35-2, STX4A

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38156

Provider

Invitrogen Antibodies

Product name

Syntaxin 4 Recombinant Rabbit Monoclonal Antibody (8F3P7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Samples: A-549, Mouse lung, Rat brain Immunogen sequence: QTIVKLGNKV QELEKQQVTI LATPLPEESM KQELQNLRDE IKQLGREIRL QLKAIEPQKE EADENYNSVN TRMRKTQHGV LSQQFVELIN KCNSMQSEYR E

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

8F3P7

Vial size

100 µL

Concentration

1.3 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of Syntaxin 4 in NIH-3T3 cells using Syntaxin 4 Recombinant Monoclonal Antibody (Product # MA5-38156) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Syntaxin 4 in NIH-3T3 cells. Samples were incubated with Syntaxin 4 Monoclonal antibody (Product # MA5-38156) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Syntaxin-4 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of STX4 recombinant monoclonal antibody (Product # MA5-38156) with 30 µL of protein A-Sepharose beads and rocked overnight at 4 deg. 500 µg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4 deg. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using the same antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of Syntaxin-4 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of STX4 recombinant monoclonal antibody (Product # MA5-38156) with 30 µL of protein A-Sepharose beads and rocked overnight at 4 deg. 500 µg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4 deg. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using the same antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.