PA5-118290

antibody from Invitrogen Antibodies

Targeting: STX4 p35-2, STX4A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118290

Provider

Invitrogen Antibodies

Product name

Syntaxin 4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Syntaxin 4 Polyclonal Antibody (Product # PA5-118290) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Syntaxin 4 Polyclonal Antibody (Product # PA5-118290) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Syntaxin 4 in U251MG cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Syntaxin 4 Polyclonal Antibody (Product # PA5-118290, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of Syntaxin 4 in U251MG cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Syntaxin 4 Polyclonal Antibody (Product # PA5-118290, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Syntaxin 4 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 µL with Syntaxin 4 Polyclonal Antibody (Product # PA5-118290) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Syntaxin 4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.