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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- NBP2-61621 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLE3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specificity of human TLE3 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEK 293
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLa nuclear extract
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLE3 Antibody (CL3573) [NBP2-61621] - Western blot analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human cervix shows strong nuclear immunoreactivity in epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human small intestine shows moderate nuclear immunoreactivity in glandular epithelium cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human oral mucosa shows strong nuclear positivity in epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human tonsil shows nuclear immunoreactivity in a subset of lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human kidney shows weak to moderate nuclear positivity in a subset of cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLE3 Antibody (CL3573) [NBP2-61621] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
