500-P124-100UG

antibody from Invitrogen Antibodies

Targeting: CCL23 Ckb-8, CKb8, MIP-3, MPIF-1, SCYA23

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P124-100UG

Provider

Invitrogen Antibodies

Product name

CCL23 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.

Concentration

0.1-1.0 mg/mL

References

Submitted references

Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization.

Jha AK, Huang SC, Sergushichev A, Lampropoulou V, Ivanova Y, Loginicheva E, Chmielewski K, Stewart KM, Ashall J, Everts B, Pearce EJ, Driggers EM, Artyomov MN
Immunity 2015 Mar 17;42(3):419-30

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis CCL23 Polyclonal Antibody (Product # 500-P124-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL23 Polyclonal Antibody (Product # 500-P124-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL23 Polyclonal Antibody, Biotin (Product # 500-P124BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23).