500-P124-100UG
antibody from Invitrogen Antibodies
Targeting: CCL23
Ckb-8, CKb8, MIP-3, MPIF-1, SCYA23
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- 500-P124-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL23 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). Anti-Human MIP-3 (CCL23)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3 (CCL23) matrix. Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23). Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization.
Jha AK, Huang SC, Sergushichev A, Lampropoulou V, Ivanova Y, Loginicheva E, Chmielewski K, Stewart KM, Ashall J, Everts B, Pearce EJ, Driggers EM, Artyomov MN
Immunity 2015 Mar 17;42(3):419-30
Immunity 2015 Mar 17;42(3):419-30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis CCL23 Polyclonal Antibody (Product # 500-P124-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL23 Polyclonal Antibody (Product # 500-P124-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL23 Polyclonal Antibody, Biotin (Product # 500-P124BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23).