MAB371

antibody from R&D Systems

Targeting: CCL23 Ckb-8, CKb8, MIP-3, MPIF-1, SCYA23

Western blot (Recommended by provider)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB371

Provider

R&D Systems

Product name

Anti-Human CCL23/MPIF-1 Monoclonal Antibody (Clone 69512)

Antibody type

Monoclonal

Antigen

E. coli-derived recombinant human CCL23/MPIF-1, Arg22-Asn120

Description

Protein A or G purified from ascites

Reactivity

Human

Host

Mouse

Antigen sequence

P55773


Isotype

IgG

Vial size

500 µg

References

Submitted references

CCL23 expression is induced by IL-4 in a STAT6-dependent fashion.

Novak H, Müller A, Harrer N, Günther C, Carballido JM, Woisetschläger M
Journal of immunology (Baltimore, Md. : 1950) 2007 Apr 1;178(7):4335-41

---

CCL23 expression is induced by IL-4 in a STAT6-dependent fashion.

Novak H, Müller A, Harrer N, Günther C, Carballido JM, Woisetschläger M
Journal of immunology (Baltimore, Md. : 1950) 2007 Apr 1;178(7):4335-41

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL23/MPIF-1 and Neutral-ization by Human CCL23/MPIF-1 Antibody. Recombinant Human CCL23/MPIF-1 (Catalog # 371-MP) chemoattracts the THP-1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF-1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF-1 Monoclonal Antibody (Catalog # MAB371). The ND₅₀ is typically 2-6 µg/mL.