SM3127P

antibody from OriGene

Targeting: SYK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

SM3127P

Provider

OriGene

Product name

SYK (5-360) mouse monoclonal antibody, clone SYK-01, Purified

Antibody type

Monoclonal

Description

SYK (5-360) mouse monoclonal antibody, clone SYK-01, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

SYK

Isotype

IgG

Antibody clone number

SYK-01

Vial size

100 µg

Concentration

1.0 mg/ml

References

Submitted references

A novel lipid raft-associated glycoprotein, TEC-21, activates rat basophilic leukemia cells independently of the type 1 Fc epsilon receptor.

Hálová I, Dráberová L, Dráber P
International immunology 2002 Feb;14(2):213-23

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blotting analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01). Lane 3, 4: immunostaining with anti-human Cytokeratin 18 (DC-10; SM1360PS). Lane 5: negative control

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence staining of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence staining of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Validation comment

IF