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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0623 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0623, RRID:AB_982957
- Product name
- SYK (phospho Y525/Y526) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of SYK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An IgM class anti-neutrophil cytoplasm antibody inhibits neutrophil adhesion and apoptosis via a Syk dependent signaling cascade.
Hypermethylation of Syk gene in promoter region associated with oncogenesis and metastasis of gastric carcinoma.
T-cell receptor-induced phosphorylation of the zeta chain is efficiently promoted by ZAP-70 but not Syk.
Hayes MJ, Cambridge G
Molecular immunology 2004 Jun;41(4):457-68
Molecular immunology 2004 Jun;41(4):457-68
Hypermethylation of Syk gene in promoter region associated with oncogenesis and metastasis of gastric carcinoma.
Wang S, Ding YB, Chen GY, Xia JG, Wu ZY
World journal of gastroenterology 2004 Jun 15;10(12):1815-8
World journal of gastroenterology 2004 Jun 15;10(12):1815-8
T-cell receptor-induced phosphorylation of the zeta chain is efficiently promoted by ZAP-70 but not Syk.
Steinberg M, Adjali O, Swainson L, Merida P, Di Bartolo V, Pelletier L, Taylor N, Noraz N
Blood 2004 Aug 1;104(3):760-7
Blood 2004 Aug 1;104(3):760-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SYK (phospho Y525/526) polyclonal antibody (Cat # PAB0623) in mouse liver tissue lysate (35 ug/lane). Phospho-SYK (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SYK (phospho Y525/526) polyclonal antibody (Cat # PAB0623) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
