H00006850-D01P

antibody from Abnova Corporation

Targeting: SYK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006850-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006850-D01P, RRID:AB_1581173

Product name

SYK purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human SYK protein.

Antigen sequence

MASSGMADSANHLPFFFGNITREEAEDYLVQGGMS
DGLYLLRQSRNYLGGFALSVAHGRKAHHYTIEREL
NGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKK
PFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQG
QALEQAIISQKPQLEKLIATTAHEKMPWFHGKISR
EESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHE
GKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYS
YKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSH
PATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQE
STVSFNPYEPELAPWAADKGPQREALPMDTEVYES
PYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTV
KKGYYQMKKVVKTVAVKILKNEANDPALKDELLAE
ANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGP
LNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNF
VHRDLAARNVLLVTQHYAKISDFGLSKALRADENY
YKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGV
LMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPA
GCPREMYDLMNLCWTYDVENRPGFAAVELRLRNYY
YDVVN


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SYK expression in transfected 293T cell line (H00006850-T01) by SYK MaxPab polyclonal antibody.Lane 1: SYK transfected lysate(72.10 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SYK MaxPab rabbit polyclonal antibody. Western Blot analysis of SYK expression in HeLa.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between SYK and CTTN. HeLa cells were stained with anti-SYK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)