Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 17-6696-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Syk Monoclonal Antibody (4D10.1), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 4D10.1 monoclonal antibody recognizes human Syk, a 72-kDa member of the Syk/ZAP-70 family of non-receptor protein tyrosine kinases. Syk is expressed most highly in B cells, with lower expression in immature thymocytes, mast cells, and platelets. Syk can also be detected in fibroblasts, epithelial cells, hepatocytes, endothelial cells, and neuronal cells. This protein is a major component of signaling cascades downstream of the B and T cell antigen receptors and plays an essential role in lymphocyte development. Upon recruitment to immunoreceptor tyrosine-based activation motifs (ITAMs) on the antigen receptors, Syk is activated by phosphorylation on multiple tyrosines. Once activated, Syk phosphorylates proteins such as phospholipase C gamma and BLNK/SLP-65. Finally, abnormal Syk expression has been linked to tumor cell migration and invasion in several cancers.
- Antibody clone number
- 4D10.1
- Concentration
- 5 µL/Test
Submitted references Specific human cytomegalovirus signature detected in NK cell metabolic changes post vaccination.
Woods E, Zaiatz-Bittencourt V, Bannan C, Bergin C, Finlay DK, Hoffmann M, Brown A, Turner B, Makvandi-Nejad S, Vassilev V, Capone S, Folgori A, Hanke T, Barnes E, Dorrell L, Gardiner CM, PEACHI Consortium
NPJ vaccines 2021 Sep 28;6(1):117
NPJ vaccines 2021 Sep 28;6(1):117
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) followed by intracellular staining with Mouse IgG2a K Isotype Control PE (Product # 17-4724-81) (left) or Anti-Human Syk APC (right) using the Foxp3 Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 HCMV impacts on functional and metabolic responses of CD56 dim NK cell subsets post vaccination. Summary of canonical and adaptive CD56 dim NK cells responses at baseline and post-priming vaccination (Day 0, Day 7 and Day 28). Canonical (FcepsilonRgamma1+Syk+) or adaptive (FcepsilonRgamma1+Syk-, FcepsilonRgamma1-Syk+ and FcepsilonRgamma1-Syk-) cells were analysed as indicated for a ex vivo frequencies of NK subsets, b frequency of pS6+, c IFNgamma+ and d IFNgamma MFI in response to IL-12/IL-15 stimulation after vaccination ( n = 4). e Summary of post-priming Mitotracker and f ATP5B MFI in canonical and adaptive CD56 dim NK cells at baseline and Days 7 and 28 ( n = 4). Error bars show SEM. a , e , f Samples were compared by mixed-model with Bonferroni post hoc test. b - d Samples were compared by two-way ANOVA with Sidek's post hoc test. n.s. not significant.